Wednesday, July 31, 2019

Wuthering Heights-the Structure and Style Transcend the Time

Wuthering Heights —— The Structure and Style Transcend the Time Emily Bronte describes the principal human conflict as one between the individual and the dark, questioning universe, a universe symbolized, in Wuthering Heights, both by man’s threatening and inner nature, and by nature in its more impersonal sense, the wild lonesome mystery of the moors. The structure and narrative style of Wuthering Heights transcend her time. Emily didn’t follow the regular and secular romantic writing techniques at that time, in contrast, she surpassed and created some new skills which modern readers are apt to understand and love. In this article, you will find the following six aspects of the writing style, including the approach of the story’s center step by step with spiral circle, the end echoes the beginning, symbolism of the two generations, the love which makes the negative turn to be positive, the dark satanic hero, and the alternant emotion with contradiction throughout the whole story. Emily has written a novel which seeks to move ever closer to the center of a unique and remarkable human relationship, and the very structure of her book emphasizes this movement. In Chapter One, for example, readers are as far as possible from the heart of the story’s experience due to the innocent guesses of Lockwood (the narrator or diarist). However, later chapters move progressively closer to the heart of the story, and the beginning, with its subtle suggestions of an old tragedy and with Lockwood’s naive judgments of Heathcliff, will come to be prophetic. Therefore, the plot of Wuthering Heights is not sequential and linear, but described as a spiral circle compared with other coetaneous novels. That is to say, readers have been always following Lockwood to circle around the center of the story step by step, until the origin of the tragedy vividly comes in front of their eyes. In spite of the spiral circle, the end echoes the beginning, which is another exquisite narrative technique, capture readers’ hearts. The last chapter tells of Heathcliff’s slow disintegration and death. The death itself is preceded by a fasting, reminiscent of the fasting which precipitated Catherine’s final illness. Because Heathcliff is described as robust and healthy shortly before his sudden decline, we suppose that it is his overwhelming desire or will to die and to return to his beloved Catherine, the thought of which â€Å"lights his face with a strange joy†(Wuthering Heights 137) for days, that really kills him, and not the mere abstinence from food. The structure of the book achieves an almost perfect symmetry in the death of Heathcliff. And the end of the novel as at the beginning, the master spirit is staring out into a storm, searching for Catherine. Emily Bronte features similar destiny about the two generations, but different endings of them. She describes this kind of symbolism by giving the names Catherine and Linton. Both Catherine (Catherine Earnshaw and Catherine Linton) marries Linton (Edgar Linton and Linton Heathcliff), who they don’t love most. The mother Catherine dies before the book is half over, but her spirit continues to rage in the turbulent air of Wuthering Heights, haunting Heathcliff, and also returns, healthily subdued, in her daughter Cathy. The daughter finally gains happiness which stretches over two generations. And we may say that these two Catherine can be considered as one person who is also the heroine throughout the whole story. The other is about Linton. Linton Heathcliff, who is â€Å"a nervous, sickly, effeminate child, weak-willed and petulant like his mother, and, like her, the pitiful victim and tool of his father† (Wuthering Heights 112)inherits disadvantages from both sides of his parents——the peevishness and self-pity of the mother and the bad temper of the father. It is ironic but the symbolism is clear. Hate is barren. Contrast to hatred, the love in this novel is also particular. Heathcliff and Catherine suffer from the separation for many years even after the heroine’s death. Only death can bring them together because of those insurmountable social and conceptual gaps even though Catherine’s nature is â€Å"a nature that is one with Heathcliff’s† (Wuthering Heights 58). Heathcliff's whole life is an embodiment of the force of evil. Contemplating his history is like peering at a beloved film of a picture: everything that should be dark is fading and everything that should be light is covered with darkness. Heathcliff and Catherine love each other by inflicting pain on one another instead of permitting pleasure. They did not live together when they were alive; they could love together after they died. They sustained themselves not by eating but by refusing to eat. It is Emily Bronte’s triumph as novelist that as her book proceeds, the negative becomes positive. Even in the end, through the rumors, â€Å"Catherine and Heathcliff walk the moors at night and even appear within the house at Wuthering Heights. (Wuthering Heights 140) They finally get together after they died and the negative death turns to a positive and extricable ending. And about the typical hero of the novel, Heathcliff is one of the most attractive characters in the history. To answer the question why he is so popular, it should be attributed to the black description of this â€Å"dark Satanic† hero. â€Å"Heathcliff, of course, is frequently compared to a demon by the other characters in the book. † (Sparknotes: Literature Study Guides: Wuthering Heights: Analysis of Major Characters) At first glance he may seem entirely wicked, even a criminal. The vicious way in which he destroys Hindley and brutalizes Isabella suggests that he is a man for whom sympathy ought to be impossible. Yet Emily Bronte manages her dark hero a sympathetic figure. When he has gone so far as to drive Lockwood out into the storm alone, there comes one of the overwhelmingly lyric moments in the novel as Heathcliff leans far out of the window and implores the spirit of Catherine to come in. The depth of feeling, the compassion of which Heathcliff is plainly capable in this scene, forces us to reconsider our judgment of the man. Without question he is brutal, but just as plainly he has within him the potential for great tenderness and love. Obviously, this potential has been destroyed somewhere along the line, and those readers, their interest aroused in how this could have happened, read on. Besides the hero, Heathcliff, who we love to hate, the fluctuation of alternant emotion is also a point of contradiction throughout the novel — — violent but dreamlike, brutal but romantic, fanatical but gloomy, all of these consist of a piece of deserted wilderness with mysterious beauty. Readers easily feel lost into the anxiety and disturbance that Emily delivered to them, as well as the desire to explore the ins and outs of the whole story under an intangible force. And the last paragraph that Lockwood said to himself in the end of the novel gives readers a peaceful and harmonious aftertaste despite of all the thrilling revenge and love. â€Å"Under that benign sky; watched the moths fluttering among the heath and harebells; listened to the soft wind breathing through the grass; and wondered how anyone could ever imagine unquiet slumbers for the sleepers in that quiet earth. The metaphor here is about the hard â€Å"heath†, which is a part of Heathcliff, comparing with soft wind, in order to leave hope for readers. The six aspects above can be divided into two parts: the structure and the characters which of both have been created surpassing the time of Emily Bronte. On one hand, the spiral circle and the correlation between the end and the beginning, is th e key to the structure of the novel. The book begins in 1801, on the very rim of the tale, long after the principal incidents of the story have taken place. Readers are far from the heart of the novel in the first pages, however, blundering along with the guide Lockwood later. Gradually we spiral in toward the center. But neither Lockwood nor Dean is unperceptive and we must struggle hard before we can actually achieve the true center of the novel, the passionate last meeting of Heathcliff and Cathy in which, for a moment, we are permitted to stare into the heart of the fiery furnace. On the other hand, about the most powerful character in the novel, the darkness and violence that was in Heathcliff from the beginning, is in every man. And because this darkness is so primal and so universal, it can never be overcome. It persists, implacable and unchangeable, a comment not just on one man’s special sorrow but on every man’s dark heritage. That is why a dark Satan is more attractive than a pure Angel in readers’ hearts. And Heathcliff is a powerful figure not only because he is rooted in the traditions of his own time, from which he draws strength, but also because he makes a universal statement about man’s nature, which continues to strike readers today as remarkably fresh and modern. Therefore, no matter the structure or the character that attracts lots of readers in history, the remarkable sense of the privacy of human experience, is clearly the central vision of Wuthering Heights and it is always being transcending the time. Works Cited Bronte, Emily, Wuthering Heights, Beijing: Foreign Language Teaching and Research Press, 2005 Gui Tuoqing, Selected Readings in English and American Literature, Beijing: China Foreign language Translation Press, 1985 Wuthering Heights, http://www. sparknotes. com/lit/wuthering/canalysis. html , , : ,1994 , , : ,2000

Tuesday, July 30, 2019

Match made in heaven Essay

â€Å"There is nothing nobler or more admirable than when two people who see eye to eye keep house as man and wife, confounding their enemies and delighting their friends. † Homer That bard created such two people in The Odyssey, their contrasting roles concealing the similarities in their natures. Both Penelope and Odysseus dealt with â€Å"a world of pain†, but in very different settings: she wastes away at home, while he faces a myriad of adventures and sufferings around the Greek world. Although Homer assigned them dissimilar parts in his epic, however, his story still reveals striking resemblances between Odysseus and Penelope: they possess positive qualities and several faults in common as well as one major dissimilarity, all of which are the secrets to their long and blissful marriage and help them to see â€Å"eye to eye. † One can easily see why Ithaca’s king and queen remained happily united for so many years when looking at the shining characteristics they share. Both are wondrously loyal, even when faced with an abundance of temptations. Over the course of twenty years, Odysseus knew countless lovely women, from Nausicaa to Calypso, yet he remained determined to return to his wife. Likewise, Penelope had her choice of one-hundred and eighty of the best men in Greece all vying for her hand, but she still â€Å"falls to weeping for Odysseus† every time she thinks of her beloved husband. Undoubtedly, The Odyssey’s happy ending could not have occurred without their mutual fidelity. Cunning brilliance is the second attribute common to both Odysseus and Penelope, and it served to reunite them as much as their reciprocal devotion did. Odysseus is known as the â€Å"man of twists and turns†, and presumably, he used his acumen to select a wife who could match him in matters of the mind. Being the hero of the story, Odysseus’s brains are flaunted by Homer in his every action, from his escape from the Cyclops to all the creative stories he fabricated. But Penelope’s wisdom can also be detected within the text, and is crucial to the plot. For example, take the often-retold story of her web, woven and unwoven to keep the suitors at bay for three years, or when in Book 18, she coyly elicited expensive gifts from each suitor to compensate for some of her husband’s squandered estate. One can also adduce the test she devised for the suitors as a confirmation of her sagacity: â€Å"The hand that can string this bow with the greatest ease†¦ he is the man I follow. † Penelope knows very well that it is highly unlikely that one of her brazen suitors could muster the strength needed to shoot â€Å"his polished bow†: it was just another clever way postpone marriage. Had Penelope not â€Å"sp[un] out her wiles†, much like her husband had done abroad, the lovely queen of Ithaca would probably have been coerced into an unwanted union long before Odysseus returned. Not only are the queen and king of Ithaca alike for possessing dominant traits of loyalty and astuteness, they also share several shortcomings. Firstly, although they are devoted enough to pine for each other for two decades, neither were one hundred percent loyal to their spouse. Odysseus did not remain faithful to Penelope, sleeping with Circe, then Calypso, and perhaps some mortal women unworthy of being mentioned as well. Odysseus claims that he lay with the Circe for the sake of diplomacy, but if so, then why did he stay in her â€Å"arching caverns† for over a year, leaving only at his crew’s urging? Homer also hints at Odysseus’s voluntary treachery during his seven-year detainment with Calypso, including lines such as â€Å"they lost themselves in love†, and â€Å"since the nymph no longer pleased. † Is the bard implying that the nymph with lovely braids once pleased him, and thus, he willingly copulated with her? Penelope, being a woman, could not have had such affaires d’amour and still be considered loyal. However, because she was only a woman, she still harbored an innate desire to attract men. In Book 18, she fulfilled Athena’s wish that she should â€Å"display herself to her suitors, fan their hearts, inflame them more† in order to receive the suitors’ gifts, but perchance also to satisfy her own longing for attention – after all, Penelope is a woman whose husband has been gone for more than twenty years. She succeeds in accomplishing both: After she descends the stairs, â€Å"the suitor’s knees went slack, their hearts dissolved with lust† and they showered her with â€Å"gorgeous presents. † Homer seems to reiterate this fact that Penelope enjoyed the courtship of so many fine, young princes, even though her suitors were a burden and a plague to the household. Book 19 includes a passage describing a dream Penelope had, in which an eagle, which later reveals himself as Odysseus, destroys her flock of geese by â€Å"snap[ping] their necks and kill[ing] them one and all†, the geese obviously symbolizing her gaggle of suitors. Penelope is comforted by this dream and seems to hope that it foreshadows future events, but also acknowledged that she â€Å"wept and wailed† and was â€Å"sobbing, stricken† at the slaying of her geese. Hence, though Penelope does remain honorable and is faithful to Odysseus during the twenty years when they were apart, she still, perhaps subconsciously, fostered a desire to do otherwise. However, Penelope’s slight interest in her suitors may not be a have been such a bad thing; on the contrary, it could have inspired her to forgive her husband more easily if he ever told her of his dalliances with goddesses. Second, both Odysseus and Penelope are characterized as â€Å"wary†, and although their caution helped them to succeed in many situations, both are overly circumspect at times, causing them to be suspicious of those who love them most. One of the most tense and heartbreaking scenes in the book takes place in Book 23, when Penelope is face to face with her devout husband for the first time after twenty years of separation, yet refuses to acknowledge him, prompting Telemachus to reproach: â€Å"What other wife could have a spirit so unbending? Holding back from her husband, home at last for her After bearing twenty years of brutal struggle- Your heart was always harder than a rock! † Her son is right, but Penelope still refused to speak to Odysseus, even after Telemachus’s rebuking, causing the great-hearted Odysseus to â€Å"blaze up in fury† over his wife’s distrust. An analogous incident took place later between Odysseus and his old father. Seeing Laertes in the orchard, Odysseus observed him sitting alone, â€Å"his heart racked with sorrow†, a sight enough to make even â€Å"long-enduring Odysseus† stop to weep. Yet, even so, Odysseus decided to test the old man first, to â€Å"reproach him with words that cut him to the core. † It was wholly unnecessary to verify the loyalty of Laretes, for after all, the man is his father and if that were not enough, Odysseus had heard testimonies to Laretes’s grief from Eumaeus as well as his own mother in Hades. Odysseus’s often-praised caution prompted him to be rather irrational his handling of the situation, telling a tale that causes his dear father to grieve even more: â€Å"Both hands clawing the ground for dirt and grime, he poured it over his grizzled head, sobbing, in spasms. † His suspicion inflicts unnecessary pain on his father, much as Penelope’s caution angered him; nevertheless, it is because their minds operate in such a similar fashion that they are able to understand each other’s rash actions, caused by that extreme â€Å"wariness† which dominates their reasoning. It was because of their faults that Odysseus and Penelope could see â€Å"eye to eye. † But for all their innate likenesses, one main difference remains between the hero and heroine of The Odyssey: Odysseus has pride, a kind of virile self-regard that Penelope surely lacks, for better or for worse. Odysseus’s excessive self-respect gets him into many difficult circumstances: his odyssey of misery would not have occurred had he not revealed his identity to the Cyclops because he could not bear being remembered as â€Å"Nobody†. One cannot imagine that Penelope would find ever herself into such a predicament. However, there are some instances during the plot of The Odyssey when Penelope should have displayed more dignity. Several times throughout the story, Telemachus scolds her in a disrespectful manner, telling her to â€Å"Tend to your [Penelope’s] own tasks†, declaring that he â€Å"hold[s] the reins of power in this house. † Penelope didn’t put her teenage son in his proper place, opting instead to meekly withdraw to her own quarters. Had Telemachus spoken similar words to his father, Odysseus’s ego certainly wouldn’t have allowed such a lack of reverence, and Telemachus most likely would have received some tough love at his hands. Pride is the only significant distinction between the characters of Odysseus and Penelope, but it is a meaningful difference as well. If Ithaca’s queen was as self-righteous as its king, one could expect many royal family squabbles instead of the marital bliss they are famed for. Penelope is modest and demure, attributes that are absolutely necessary in order to live in harmony with a proud man like Odysseus. In conclusion, Odysseus and Penelope are alike in almost every respect, with their good qualities holding them together, their faults leading to understanding, and their one dissimilarity producing compatibility. That is why they were able to build the strongest kind of love, the love described in I Corinthians 13:7 : † Love never gives up, never loses faith, is always hopeful, and endures through every circumstance. † The Odyssey itself is a testimony to this kind of bond between husband and wife, a bond forged by harmonious natures, able to survive through twenty years of separation, temptation, and suffering.

Allocating Scarce Resources and Alternative Medicine Essay

1. Sympathy Rule: Impartiality rule: â€Å"health care professionals should usually be impartial in helping patients† (p. 189) But the rules used must be appropriate for people with ordinary human sympathies and feelings, example, biases toward friends or relatives. So the rules should allow for some bias – It was okay for Alice to send her aunt in first. Argument for the sympathy rule * The sympathy rule would not permit HC providers to help their relatives/friends in any way they like. It would permit them to give priority to their relatives only in very extreme, life and death situations. * From society’s point of view this rule is okay b/c society values both impartiality and close ties between relatives and friends. The sympathy rule is a kind of compromise between the two. Objection to the sympathy rule * We know from experience that people will already be biased toward their relatives and friends, and will have a tendency to give them priority where possible. * So, to counteract this innate bias, there must be a very strict rule against nepotism. However difficult it may be to be completely impartial as between your mother and a perfect stranger, this is what morality requires of HC professionals. 2. Innocents First Rule: Was Alice justified in sending her innocent aunt in before Terrance who caused the accident by his negligence? â€Å"Patient A, who is innocent and who cannot wait for care, ought to be given priority over patient B, who is known to be at fault for the injury and who also cannot wait, other things equal.† (p. 206) Third Party Interests 1) Childless person vs. parent of young child (dependents case) 2) Drunk vs. doctor (the public good case) 3) Person with highly infectious disease vs. person without infectious disease (the innocent threat case) 3. Public Interest Rule â€Å"Where the public interest is great enough, a patient may be given priority over those who came earlier.† (p. 208) Example, treat the Pope or the Prime Minister before the ordinary person. Discussions: 1. My answer to your question is this: No, it is not ethical to use non medical â€Å"social worth† factors to favor one patient over another. The use of such a criteria to choose between candidates for scarce medical resources can be critiqued both from Kantian and Utilitarian perspectives. I believe the most basic and powerful objection to â€Å"social worth† is that such a criteria reduces patients to their potential for maximizing social benefit. Kantian ethics argue that people should never be treated merely as a means to an end. By selecting between patients based solely on their potential contribution to society, they are being reduced to merely a means of achieving the most possible social good. They are not regarded as people with basic intrinsic value that goes beyond their ability to contribute to society. Any patient that does not have the ability to contribute much to society, because they are elderly, mental, or physically handicapped, automatically is unfairly at a disadvantage. On the face of it, Utilitarianism would seem to favor the use of such a criteria. Choosing people to save based on their potential future contribution would seem to produce the most net benefit. However, the physican/patient relationship would suffer greatly if patients felt that their physicians were continually looking past their personal welfare to the potential for social benefit. Distrust and suspicion would result if patients perceive that physicians were making treatment decisions based on their potential social value. Thus, over a longer period of time, the eventual erosion of the physician/patient relationship could result in less net benefit for society, a result not favored by Utilitarianism. Additionally, there are a host of other practical difficulties with appalling a â€Å"social worth† criteria. Use of such a criteria would require some type of ranking of respective social worth. How does a professional athlete rank against a priest, or an insurance salesman, or a physician. How much should patients’ personal life be involved in selection decisions? Given the pluralism of our society, development of such a list is nearly impossible. And even assuming that such a list could be compiled, whose ideas of social value should we use to make the list? Inevitably, personal bias would influence the ranking of â€Å"social worth†. Ultimatley, the use of medical factors, such as need, amount of resources required, and potential for success, should be used for patient selection, not non mendical â€Å"social worth† criteria. 2. As a physician for 30 years, practicing medicine from a perspective of â€Å"hearing the story† of the person, trying to ascertain the depth of their understanding, what motivates them to live, go on, do the unfinished, resolve those difficult relationships, be productive, make a difference, I find that those patients who have had transplants as adults have risen above the flat line of their lives and choose to see life as something to be lived and not something to be chased. â€Å"Social worth† has the unfortunate connotation of value, of something to be contributed, something that inherently gives a sense of future. We the elders decide who and again unfortunately are imperfect yet our decisions are irreversible. Committees are convenient because the decisions take on a vote situation based on the facts of the particular case. Sometimes I think that we do too much cerebrating and not enough feeling and deciding from the heart. The heart knows, where the brain has too much debris and stereotype to render fair decisions in who gets what organ. The system is flawed because we try to make sense out of something that exceeds our understanding and we forget what our hearts are telling us.

Monday, July 29, 2019

Sociology research Paper and Questions Essay Example | Topics and Well Written Essays - 1250 words

Sociology research Paper and Questions - Essay Example rime, this study proposes to examine whether increasing crime can in fact be attributed to race, or whether there may be other factors that could explain the explosion in crime. People belonging to ethnic minorities had long complained of racial profiling by law enforcement personnel, which is often the cause of feelings of resentment, hurt and an increasing loss of trust in the police among members of racial minorities. But Kleinig and Risse (2007) have discussed racial profiling in the context of the September 11, attacks, and detailed the results of a study that appeared to justify the evils of racial profiling as an efficient law enforcement strategy. The reason was because it was based upon the underlying belief that members of certain groups appear to possess a greater tendency to commit crimes and police can curb crime by adopting harsher measures against members of such minority groups. Based upon the findings of this study, an argument is offered that racial profiling is attributable to pre existing racism, hence profiling only expresses that racism rather than perpetuating it. Another argument offered is that the benefits of profiling and benefits provided by Government to the minorities far outweigh the harmful effects. Huff (2007) reports on an extensive ethnographic study that was carried out among police officers to examine their attitudes towards gang related crimes, their reasons for wanting to be assigned to units fighting against gang related crimes and how gang units are managed. Although this was an exhaustive study utilizing multiple data sources, it was limited by the selection of four primarily southwestern cities with a higher incidence of Hispanics. The study found that officers feel more independent while functioning in gang units but there was also clear evidence of racial profiling against the minorities. Graham and Lowery (2007) examined the problem of racial profiling from another perspective – the working of the criminal justice

Sunday, July 28, 2019

The Effects of Global Economic Integration on Countries Essay

The Effects of Global Economic Integration on Countries - Essay Example There are several crucial historical pieces of evidence which point to the fact that there existed significant trade and economic relationships among civilizations of the ancient period. Every nation of the world today is engaged in commercial, political, and economic relationships with other nations and it has become the basic strategy for the progress of these nations. In the course of time, such agreements and relationships among world-nations became essential to the economic growth and prosperity of every nation, paving the way for the current international organizations and associations for regional and global agreements. This paper examines, based on empirical research findings, the effects of global economic integration on countries and the world, focusing on both the positive and negative impact of global integration. Global economic integration is an important economic process affecting various nations of the world in the age of globalization and it is essential to comprehend the basic aspects of this process. Thus, economic integration can be defined as the economic process â€Å"whereby markets and production in different countries become increasingly interdependent due to dynamics of trade in goods and services and flows of capital and technology† (Sparrow, Brewster & Harris 17). As Saskia Sassen (2006) maintains, â€Å"a good part of globalization consists of an enormous variety of micro-processes that begin to denationalize what had been constructed as national — whether policies, capital, political subjectivities, urban spaces, temporal frames, or any other of a variety of dynamics and domains’’ (Sassen, 1). Dominick Salvatore explains the theory of economic integration as â€Å"preferential trade arrangements to free trade areas, customs unions, common markets and economic unions’’ (Salvatore 321).  

Saturday, July 27, 2019

A Single American Nation Assignment Example | Topics and Well Written Essays - 250 words

A Single American Nation - Assignment Example he failures includes: Failure to achieve a massive federal works program, full and fair employment, decent housing and adequate integration education. Martin Luther King Jr. was a devoted Christian whose mission was to get his accomplishment without aggression or violence whereas Malcom X was a Muslim who wanted there to be a clear segregation of the white and black people in America because he felt that they would never coexist together. Even though King and Malcom differed majorly on racial segregation they both struggled in their own ways to bring about Africa American into a beta position in the society. Many movements had erupted 1960s including civil rights, women movements and environmental movements. These movements had emerged due to several interrelated reasons: Because American people looked upon the federal government to solve their problems, economic disparity, political competition and a moral culture that fostered unity in America. U.S. was more united in the 1970s than in the 1950s because of the remarkable achievements of all the movements especially the civil rights movements in the 1960s. This could be indicated by rights to vote for all people and integration in education and the coexistence of all races

Friday, July 26, 2019

Just in Time for Starbucks Case Study Example | Topics and Well Written Essays - 750 words

Just in Time for Starbucks - Case Study Example This paper seeks to analyze Starbucks Operations Management in the lights of broad and diverse academic resources. Recent research conducted in the market suggests that Starbucks has been unsuccessful in meeting expectations of consumers in the customer satisfaction domain. The organization needs to devise and implement a strategic plan in order to generate revenues as well as improving customer satisfaction. Market research suggests that Starbucks was considered to be the biggest coffeehouse in the United States as well as in other countries. It concentrated on younger, lower income, customers. At the same time, it is not meeting the demands of customers in the customer satisfaction domain (Thompson & Martin, 145). At Starbucks, the Senior Management is responsible for analyzing the outcomes of the market research so that it could understand the factors which led to customer dissatisfaction in order to devise plan to meet their demands and increase revenues and sales. In recent times, the demographics of conventional Starbucks have changed. Now days, younger, low income and less educated customers visit the coffee house. Research suggests that traditional customers in the past were considered to be white collar customers aged between twenty five and forty (Zeithaml, 50). Although several factors were identified which had made an impact on customer satisfaction, the speed of service and overall service have been acknowledged as the most important. The company has spent and invested huge amount of money of employee benefits in order to maintain speed of service. Competition Research suggests that Starbucks was ahead of its competitors in operations and profitability. In the United States, Starbucks have competed against several coffeehouse chains. Its competitors include Caribou Coffee, independent specialty coffee outlets, Dunkin Donuts, etc. Company Strategy and Objective The aim and objective of Starbucks is to establish itself as the most popular and famous coffee brand in the world by concentrating on expansion of retails and introducing new products. At the same time, it concentrates on creating a memorable experience around coffee consumption and to accomplish it, they have expanded and operated an experimental branding approach. Customer service was integral part of this approach. Context As mentioned earlier, Starbucks strives for innovation and keeps on investing on new products in order to generate sales and profits. The recent market research indicates that customer service was deemed as unsatisfactory because customers viewed Starbucks as a giant corporation which concentrates on making profits and expanding it self. This indicates that using customer snapshot scores were ineffective in determining the service performance (Hammonds, 245). Consequently, the senior management is worried and needs to devise a plan in order to address the issue of customer satisfaction. By investing more than forty million dollars on additional labor hours is uncertain and risky because this strategy would only concentrate on improvement of speed of service. Recommendations The company needs a wide ranging diverse and broad strategy, which concentrate on customer service. This strategy should concentrate on understanding the needs of the customers and improving their experience. With the improvement in customer satisfac

Thursday, July 25, 2019

Use of computers in construction cost controlling process Essay

Use of computers in construction cost controlling process - Essay Example Cost planning is the means that enables the objectives of project cost management to be achieved. It is the process used during the design stage of a particular scheme to help minimize the cost of construction and subsequent usage, and maximize the functionality that is anticipated by the client. Cost planning is, in fact, a system of procedures and techniques used by quantity surveyors. Its purpose is to ensure that clients are provided with value for money on their projects; that clients and designers are aware of the cost consequences of their proposals; that if they so choose, clients may establish budgets for their projects; and the designers are given advice that enables them to arrive at practical and balanced designs within budget. Therefore cost planning monitors and helps direct design and organizational decisions in order to achieve the clients cost objectives. Cost planning is a process that brings cost information to bear systematically upon the evolution, construction, and maintenance of a project to highlight the relationship between capital and operational investment, quality, function, and appearance, and to provide a framework for the management of costs in order to deliver value for money to the client.( Flangan, 1997) The cost plan is one of the principal documents prepared during the initial stages of the cost management process. It is a statement of the proposed expenditure for each section or element of a building related to a definite standard of quality. ... estimate, tender figure and final account sum are all closely related. Cost Planning : Cost planning is the means that enables the objectives of project cost management to be achieved. It is the process used during the design stage of a particular scheme to help minimize the cost of construction and subsequent usage, and maximize the functionality that is anticipated by the client. Cost planning is in fact a system of procedures and techniques used by quantity surveyors. Its purpose is to ensure that clients are provided with value for money on their projects; that clients and designers are aware of the cost consequences of their proposals; that if they so choose, clients may establish budgets for their projects; and the designers are given advise that enables them to arrive at practical and balanced designs within budget. Therefore cost planning monitors and helps direct design and organizational decisions in order to achieve the clients cost objectives. Cost planning is a process that brings cost information to bear systematically upon the evolution, construction and maintenance of a project to highlight the relationship between capital and operational investment, quality, function and appearance, and to provide a framework for the management of costs in order to deliver value for money to the client.( Flangan, 1997) The cost plan is one of the principal documents prepared during the initial stages of the cost management process. It is a statement of the proposed expenditure for each section or element of a building related to a definite standard of quality. Costs, quantities and specification details are itemized by

Wednesday, July 24, 2019

Importance and Benefits of Wetlands Essay Example | Topics and Well Written Essays - 500 words

Importance and Benefits of Wetlands - Essay Example One can imagine the importance of wetlands by the idea that these wetlands provide sources on which the ecosystems are based. These wetlands actually provide support to the entire ecosystems. There are some plants species that can only and specifically survive in the wetlands i.e. water lilies, sedges, cypress, cattails, mangroves and many more other species. These plant species, which are specific in the wetlands and can only grow in a certain environment, are the food for many other living organisms. Therefore, we can say that the wetlands provide food for certain living organisms in the form of different plants. Like plants, there are many other marine animals, which are only specific to the wetlands. These animals are also wetland specific and cannot live in any other area except wetland. The rate of moisture can be seasonal or permanent on these wetlands. Similarly, in these wetlands the water can be different depending upon the nature of wetland. It can be fresh water, salt wat er, marshes, brackish water. The water present on the wetlands can be used in different ways. Like many other things in the natural environment, the wetlands are also disturbed by the human activities. The human beings are using these wet lands for their own construction and other purposes. Another reason behind the problems with the wetlands is the increasing rate of pollution and specially water pollution. These pollution resources are actually destroying the natural habitat of many species, which are specific to the wetlands and cannot grow with a wetland area. The negative impacts of human activities are very clear on these wetlands because they are being cleared or eroded by the human beings. This erosion may destroy the natural chemical makeup of all the species, which are related to the habitat of wetlands and thus a major portion of the wetlands can be shattered.

Sex Without Love by Sharon Olds Research Paper Example | Topics and Well Written Essays - 250 words

Sex Without Love by Sharon Olds - Research Paper Example eciation of sex in modern society in its deliberate rejection of warm human to human relationships in favor of the clinical focus of professional athletes obsessed with their performance and physique. The style of the poem is deceptively naturalistic, with no rhymes or stanzas. Some key ideas are presented with alliteration, for example â€Å"wine, wet† (Olds, 1984, line 6) and â€Å"the purists, the pros† (Olds, 1984, line 14). Fourfold repetition of the phrase â€Å"come to the† in lines 9-10, into which the word â€Å"God† is placed, without any proper punctuation, breaks the flow of the otherwise unremarkable syntax, as if to suggest that the the act of making love is some kind of structural anomaly in another wise regular world. This is an example of the â€Å"proud, urgent, human voice† (Poetry Foundation, p. 1) of the poet at work. Some rather graphic bodily similes such as â€Å"faces/red as steak, wine, wet as the children at birth whose mothers are going to/give them away† (Olds, 1984, lines 5-8) break some cultural taboos such as cannibalism and the mothers who neglect their responsibilities. This is an example of what Lewallan calls â€Å"her penchant for imaging the bodies of others as meat, red and raw, as well as her penchant for metaphors of predation.† (Lewallan, 2006, p. 42) There are undertones of violence and neglect in this depiction which contradict prevailing cultural expectations. From all three of these critical perspectives, the sociological, the cultural and the stylistic, Olds presents a re-evaluation of one of the oldest poetic themes in the world: the relationship between love and sex and encourages the reader to view a new, and somewhat shocking paradigm. Lewallan, Walter E. â€Å"The signature poetics of Sharon Olds and John Cage.† Dissertation, University of Florida, 2006. Available online at:

Tuesday, July 23, 2019

Employment Business Law Coursework Example | Topics and Well Written Essays - 250 words

Employment Business Law - Coursework Example w their workers should spend their off hours from duty, the fact that the airline did not act from the previous cases of sexual harassment proves their liability in this case. Other New York torts of negligent hiring, retention and supervision of employees find the employer liable. Though the hotel rooms were not a property of the airline, the fact that they rent the hotel rooms for their employees proves their liability (England, 2012). It is evident that the woman was discriminated against because of her faith. Everyone has a right to worship in a religion of his or her wish and this should not be resulted into discrimination by others who do not believe in that religion. Thus, the court should decide that the company compensates the woman and gives her the job back. The reason is the manager and assistant manager of the store discriminated against her due to her Wiccan faith. They violated the law of religious discrimination and work situations in the title VII of the civil rights act of 1964. It states that under no circumstances should a worker be discriminated against in any aspects of hiring, pay, firing and any other aspects of employment. The manager terminated her job that inconvenienced the woman in question. It is important for an employer to give their employees a reasonable notice of termination. The fact that the store manager never formally disciplined the woman for her wearing the Wiccan medallion s howed that there was nothing wrong with it. However, they had to terminate her for allegedly missing a scheduled day at work that she was not aware of. The action was a way of eliminating the woman from the store due to her Wiccan

Monday, July 22, 2019

Nursing Medicine Essay Example for Free

Nursing Medicine Essay Medical physicians and nurses have well-built passions about their range of contributions to the health of people. They are concerned with how expert ideas and welfares are to be weighed against the necessity to maneuver a system that works for the greater good of the public. Nurses and physicians work hand in hand for the cure and betterment of their patients; but what makes them different from each other? Some say it’s better to be a doctor for one has the capability to save lives; others say it’s more fulfilling to be a nurse because you not only help offer cure for the sick, you also give them genuine care. Moreover, applying and promoting caring values in the nurses’ practice is not only necessary to their own health as nurses, but its implication is also deeply tributary to finding meaning in their work. Upholding caring values in the nurses’ every day practice helps go above the nurse from a state where nursing is thought as just a job to that of a rewarding profession. While many people see medicine as a better profession than nursing, they don’t realize that nurses also encompass the competence and deep levels of character. The changes in the delivery systems of health care around the world have intensified nurses’ workloads and responsibilities. Nurses must know how to deal with patients’ increased acuteness and intricacy with regards to their health care condition. Regardless of such hardships, nurses are able to find ways to conserve their caring practice. Giving care to different individuals several hours a day is no easy job. This paper will enlighten people to what the nursing profession really is by discussing the theory of human caring. Jean Watson’s caring theory can be seen as essential to this aim. While the sole focus of medicine is to diagnose a patient and cure his/her disorder or disease, nursing entails giving care to the patient. Without care, the cure for the disease would be possible, but the illness would still linger because health would still not be attained without caring. The core of nursing is caring, while that of medicine is cure. This paper doesn’t imply that medicine does not include caring for patients. It’s just that nursing implies caring in a deeper, more attached way. The nursing profession involves working long hours with the sick, developing rapport in every possible way, and acknowledging every patient whatever their attitude or race may be. Nursing and caring have always been thought of as acting in unison. Most people choose nursing as a line of work because of their longing to care for other persons. Watson’s caring theory not only allows the nurse to live out the art of caring, it also seeks to offer compassion to ease families’ and patients’ suffering, and to support their dignity and healing. According to Watson (2001), the chief elements of her assumption are: the carative factors, the transpersonal caring relationship, and the caring moment or caring occasion. These fundamentals are described later in the paper, and will be exemplified and viewed in light of the nursing and medical profession. The caring theory or model can also be measured as an ethical/moral and philosophical foundation for professional nursing and a division of the vital focus for nursing at the corrective level. A replica of caring includes a call for both science and art; it provides a structure that intersects and embraces with science, humanities, art, spirituality, and a new magnitude of body, mind, and spirit. Nursing and medicine is developing openly as a core to human phenomenon of the nursing practice. Carative factors are viewed as a guide for the nursing core. Watson uses the word carative in contrast with medicine’s curative factors. She uses the carative factors for the reason of honoring the human magnitude of the work of nursing and the subjective experiences and inner life world of the patients that they serve. The carative factors have ten elements: faith-hope, humanistic-altruistic system of value, expressing positive and negative feelings, helping-trusting human care relationship, transpersonal teaching-learning, creative problem solving caring process, human needs assistance, supportive, protective and corrective physical, mental, societal and spiritual environment, and existential phenomenological spiritual forces. This however evolved into the caritas process which has a deeper spiritual magnitude and dimension which means to treasure and to give exceptional loving attention (Watson, 2001). Transpersonal Caring regards the harmony of life and relations that move in concentric circles of caring from a person, to others, to the society, to the world, to our planet, and to the universe. Transpersonal caring is established in a happening or actual caring instance. It shows concern for the life within. The patient is regarded as complete and as a whole, in spite of disease or illness (Watson 2003). The transpersonal nurse looks for a connection to embrace the soul or the spirit of the patient, by way of healing and caring (Watson 2003). Caring in the nursing career takes place each time a nurse to patient contact is achieved. Unlike in medicine, some doctors may look at a patient and just prescribe a drug, after that, their deal is finished. In contrast, the nurse seeks to enter the patient’s world in order to draw closer and know the patient as a caring individual, and that it is from this epistemology that the caring of nursing begins to unfold (Schoenhofer 2002). This caring makes a big difference to the well being of each patient. Caring may happen without curing, but curing cannot take place without caring (Watson 2003). It is with that conviction that nurses are concerned for patients in the anticipation that they add to the well being or cure of that patient. Hope may be the only support an ill person has to keep their optimism. Nurses care adequately to credit that hope and hold up for the patient. They have the vision that patient is complete and whole. The one being cared and one caring are unified (Watson 1997). It’s experiencing human being connection at a deeper level than a bodily contact (Watson 2003). This connection describes how the nurse goes further than an objective evaluation, presenting concerns toward the person’s deeper and subjective meaning concerning their own health care condition. The nurse’s caring realization becomes vital for the association and understanding of the other person’s point of view. This approach highlights the exclusivity of both the nurse and the person, and also the mutuality linking the two persons, which is primary to the bond. As such, the one cared-for and the one caring cooperate in connection in mutual search for wholeness and meaning perhaps for the sacred transcendence of suffering (Watson, 2001). The term transpersonal means to go further than an individual’s own ego and the here and now, while it allows him/her to arrive at a deeper spiritual connection in promoting the patient’s healing and comfort. Lastly, the objective of a transpersonal caring association corresponds to enhancing, protecting, and preserving the person’s humanity, dignity, inner harmony, and wholeness. According to Jean Watson (1988, 1999), a caring occasion is the moment (central point in time and space) when the patient and the nurse come as one in such a way that an instance for human caring is formed. Both persons, with their exceptional and phenomenal fields, have the likelihood to move toward together in a human-to-human contract. The one being cared for and the one caring for are predisposed by the actions and choices decided within the affiliation. For Watson (1988, 1999), a unique field correspond to the person’s frame of orientation or the entirety of human experience consisting of bodily sensations, feelings, spiritual beliefs ,thoughts, goals, environmental considerations, expectations and meanings of an individual’s perceptions, all of which are based upon one’s present moment, and one’s anticipated future. Not just a goal for the cared for, Watson (1999) insists that the nurse as well needs to be conscious of her own awareness and genuine attendance of being in a caring instant with his/her patient. Furthermore, in cooperation, the one cared for and the one caring can be predisposed by the caring moment throughout the actions and choices decided inside the relationship, thus, influencing and becoming a fraction of their own life history. The caring event becomes transpersonal when it permits for the occurrence of the spirit of both, then the occasion of the moment expands the restrictions of openness and has the capability to increase human capacities (Watson, 1999). Nursing can enlarge its obtainable role, long-lasting to make offerings to health care inside the contemporary model by developing its opening health strengths and caring healing that have always been in attendance on the edges (Watson, 1999). Nursing is a caring profession that is privileged as the spirit-filled, spiritual practice that it is. I deem it is a mission for a particular spiritual human being who cares about the spirituality of other persons. The humanistic nature of nursing is reflected in the caring replica. Caring is the innermost concept in the discipline of nursing. Caring may seem simple, but it entails these many aspects and it takes a lot of effort to render this, especially to people who we don’t even know. It’s easier to diagnose a patient and just prescribe some medications; it’s another story when you try to have a deep relationship with a patient while still upholding your profession. Doctors leave it to the nurse to care for the patients, because nurses are the ones who have an encounter with them 99% of the time. They are the ones who know the patients condition, even their emotions at times. Moreover, not all patients are considerate and thankful for a nurse’s efforts. Nurses must keep their moods up and not be affected by the ups and downs of their tiresome profession. At the end of the day, they still hold their faces up high and continue caring for every other patient they will be encountering in the future. Caring entails love and loving is not an easy thing to do. It is said that while a nurse enters into a patient’s room, a compelling field of expectation is produced. In this deeper, more stretched out way of thinking about the power, energy, and beauty of love, a caring moment (Watson, 1999b) becomes a vibrational field of cosmic love full of life that radiates mutuality and reciprocity, which transcends space, time, and physicality sustaining and confirming our humanity and our association with the Levinas. The infinity of the whole universe (Quinn, 1992). This ethic of caring and loving becomes the first philosophy for sustaining and facing the infinity of the nursing profession. If nurses follow this ethical order, nursing has a serious role in moving humanity in the direction of the omega point, ever closer to God and the unexplained blessed circle of loving, living, dying, trusting, and being. Lastly, a quote to ponder on the care illuminated by the nursing profession in contrast with that of medicine: â€Å"The heart is as broad as the sky, because it can embrace joy and pain side by side. † Even in the midst of hard to deal with patients, nurses still choose to care.

Sunday, July 21, 2019

Cell Culture and Protein Detection

Cell Culture and Protein Detection Overview: This practical is split into three main parts, each part allows us to develop certain techniques. The first part of the practical is focused on the techniques needed to successfully carry out cell cultures. The second part is using an analytical technique known as ELISA this is used to measure the amount of proteins secreted by the cells that were cultured in part one. Lastly, part three focuses on western blotting, this is a technique used to measure proteins too but it differs from ELISA in that it measures proteins that are present inside the cell. The fundamental objective of this practical is to look at how Bacterial lipopolysaccharide (LPS) alters the growth and the expression of the smooth muscle cell ÃŽ ±-actin (SMA) in vascular smooth muscle cells (vSMC) while also detecting and quantifying cell signalling molecules (JNK’s) and cytokine secretion (TNF-ÃŽ ±) in vascular cells. Section 1 – Subculture Objective: The aim of this part of the practical is to subculture the bovine aortic vascular smooth muscle cells and to count the cells and check for viability by counting the cells in suspension on a haemocytometer using a microscope. The cells are also treated with a bacterial antigen (LPS) to look at its effect on viability, differentiation state and the activation of intracellular signalling and secretion of proteins. Immunocytochemistry is also carried out in this part of the practical. Method: Trypsin is used to remove the cells from the bottom of the flask. When the cells have dissociated, medium which contains a trypsin inhibitor is added. The medium stops excessive trypsin action from damaging the cells. The cell suspension is first diluted using trypthan blue to allow viable cells which remain white to be distinguished from non- viable cells which are blue. This method allows us to get a total cell count of cells/mL and the percentage of viable cells. Our total cell count was found to be 7.45105 cells/mL The non-viable cells were found to be 1.5104 cells/mL This means that the culture contains more 98% viable cells which indicates that it is a healthy culture. 3 different 6 well plates were then set up using different densities of seed cells. 3105cells/ml seeding density was used in one plate. This density was used as a lot of protein needs to be present for the assessment of JNK and ÃŽ ±-actin protein cytokine production. 1105 cells/ml seeding density was used in another 6 well plate. These plates are used to assess the effect of LPS on growth and viability of the cells. This density is used to give a clear picture of the cells to make counting easier. 5103 cells/ml seeding density was used in the last plate. This plate is used for ÃŽ ±-actin expression by immunocytochemistry. The low density will give us a clearer picture. The three different sets of plates are all treated with varying concentrations of LPS. Each plate has two wells which are used as controls, containing no LPS, two wells containing 1Â µg of LPS and two wells containing 10Â µg of LPS. Immunocytochemistry is carried out on the plate with a seeding density of 5103cells/ml. This technique is used to determine if a particular protein or antigen is present. An unlabelled primary antibody is used to bind to the antigen desired antigen. The presence of contractile protein ÃŽ ±-actin can determine the differentiation state of vSMC. Immunocytochemistry is used as a qualitative method of determining the presence of a protein, it is not quantitative. Results: Calculations for different seeding densities: Initial concentration = 7.45105 cells/mL Formula = Plate 1, seeding density 1.5105 cells/ml Plate 2, seeding density 0.5105 cells/ml Plate 2, seeding density 2.5103 cells/ml Table 1: Cell growth for each group Immunochemistry results: Figure 1: Cell growth in the control (0Â µg/ml LPS) Figure 1: Cell growth in the control (1Â µg/ml LPS) Figure 3: Cell growth with 10Â µg/ml of LPS Discussion: The first part of this section was to carry out a cell count and determine the viability of the suspension. Our sample had 7.45105 cells/ml and it contained more than 98% viable cells. This meant it was a healthy cell suspension and it was suitable to run tests on for the practical. A seeding density of 1105 cells/ml seeding density was used to assess the effect of LPS on growth and viability of the cells. This density was used to give a clear picture of the cells to make counting easier. The results of our experiment correlated with the majority of the class. However there were some unexpected results in some of the groups but this could be down to plates being labelled incorrectly or mistakes while counting when using the haemocytometer. Immunocytochemistry is used to allow us to analyse the results visually. We found that as the amount of LPS increased the number of cells decreased, this is illustrated in figures 1, 2 and 3. LPS is an endotoxin and it inhibits the growth of ÃŽ ±-a ctin. LPS also damages the structure of ÃŽ ±-actin, high levels of LPS stop the correct formation of the filaments and so affects the function of the cell which is to facilitate cell contraction and migration. This result was expected as LPS is a major mediator to septic shock and is known to directly affect vascular smooth muscle cells. Question: What could you do to improve this experiment? To improve this experiment I would use a wider range of concentrations for LPS. This would give a better understanding of its affects. Repeating the experiment several times and getting an average of your results would also help. Section 2 – ELISA Objective: The aim of this section of the practical is to use Enzyme-Linked Immunosorbent Assay (ELISA) to detect production of the cytokine TNFÃŽ ± from the cells activated with LPS. Detection is based on a colour change. Firstly a standard curve must be generated so that absorbance values can be converted into concentrations of TNF-ÃŽ ±. Once the curve is completed we can determine the unknown concentrations of TNF-ÃŽ ± in our samples. Method: Firstly the antibody is immobilised onto the surface of the plate. The plate is then washed to remove any excess antibody, antigen is then added and it is allowed to bind to the antibody. A secondary antibody is then added, this antibody is labelled with an enzyme. The enzymes substrate is then added, this causes a colour change. The amount of coloured product formed is determined spectrophotometrically. The amount of coloured product is proportional to the amount of enzyme present and also to the concentration of the antigen. Results: Table. 2 Absorbance values of samples at 450nm. Figure 4. Plot of standard curve of absorbance versus concentration. Discussion: The aim of this experiment was to quantify the amount of TNF-ÃŽ ± present in our samples. To do this a set of known standards were used and their absorbance values read. This data produced a straight line with an R2 value of 0.99 indicating that a straight line was an excellent fit for absorbance versus concentration, and so the equation of the line could be used to determine unknown concentrations of TNF-ÃŽ ± based on their absorbance values. Our results showed that TNF-ÃŽ ± was present in its highest concentration of 25.15pg/ml in the sample with the highest concentration of LPS and it was found in its lowest concentration of 9.9 pg/ml in the sample containing no LPS. This result was expected as cytokines such as TNF-ÃŽ ± are produced in large quantities to respond to endotoxins such as LPS. Question: What could you do to gain more information from this experiment? To gain more information from this experiment you could test for other cytokines which are also activated by LPS, correlating these results would make your data more meaningful. Section 3 – Western Blotting Objective: The aim of this section of the practical is to prepare cell lysates from the vascular smooth muscle cells which were activated by LPS previously. SDS PAGE and western blotting will then be used to detect the activation of the intracellular protein JNK. Western analysis quantifies the amount of protein present in the cell. To do this cell lysis must be carried out. In this practical we used a method which generates whole cell lysates. To do this lysis buffer is added to the cells followed by sonication. Method: Cell lysis is carried out first. The next step is SDS polyacrylamide gel electrophoresis. Western blotting involves transferring the protein bands from an acrylamide gel to a more stable and immobilising medium such as nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out. We carried out western blotting using iBlot dry blotting system. After blotting probing is carried out to determine the presence of phosphorylated JNK protein. Results: Figure 5. Ponceau S Staining Figure 6. ÃŽ ±-actin Figure 7. pJNK Discussion: To see if our transfer was a success before probing, the blot was stained with Ponceau S stain. The proteins can be seen as red bands with this stain. The result of this stain can be seen in figure 5. red bands are present which indicates our transfer was a success and that there are proteins present. The western blot analysis showed that ÃŽ ±-actin was present in all the samples as a strong band around 42kDa was observed which is expected for ÃŽ ±-actin. The results for pJNK did not work out as it was washed incorrectly, because of this no bands were observed, however bands would be expected in the samples containing LPS. Why did you run the sample on the gel before blotting? The sample was run on gel first as proteins are separated by molecular weight. This allows us to distinguish ÃŽ ±-actin from other proteins. It is then moved to the nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out Why measure the phosphorylated form of JNK? The phosphorylated form of JNK is a signal a cell sends out when it is stressed. Therefore pJNK should be present in the samples with LPS. If it is present then it confirms the fact that the cell is stressed as a result of the presence of the endotoxin.

Separation of Amino Acids by Paper Chromatography

Separation of Amino Acids by Paper Chromatography Zarafath Jameel Table of Contents (Jump to) Introduction Applications of chromatography Types of Chromatography Paper chromatography Objectives Materials Methodology Results and Calculations The chromatogram The Rf calculated results Discussion Conclusion References Introduction Chromatography, Color written work. Chroma is a Greek roots prefix for color and graphy is a Greek roots postfix for composing. It is utilized to break down, recognize, clean measure the mixes. Chromatography is the physical division of a mixture into its individual parts The segments to be differentiated are disseminated between two stages, a stationary and versatile stage. A mixture which contains the solutes is divided into unadulterated parts by disregarding it the stationary stage (an insoluble material) to which the substances stick to fluctuating degrees. The portable stage, dissolvable is convey the solutes over the stationary stage. Partition focused around the distinctive communications of the mixes with the two stages. Substances that adhere firmly to the stationary stage move gradually, while those that stay approximately or dont stick at all move quickly. Chromatography might be a systematic strategy, in which the examiner takes in the number and nature of the parts in a little measure of a mixture, yet does not really confine them. A typical expository strategy is silica-gel dainty layer chromatography. Alternately it could be a preparative technique, in which the agent utilizes a vast amount of the mixture to get useable measures of every part. A typical preparative technique including the same stages is silica-gel segment chromatography. All chromatographic frameworks needs: A stationary stage (a robust, or a fluid upheld on a strong). a versatile stage (a fluid or a gas) sample particles (mixture for detachment) Applications of chromatography In any synthetic or bio-transforming industry, the need to partitioned and decontaminate an item from a complex mixture is a fundamental and imperative venture in the creation line. This partition of mixtures is helpful to us in different ways. As, Pharmaceutical industry utilizes chromatography to confine penicillin and different anti-toxins. Proteins can even be divided into amino acids. Chromatography is additionally utilized within wrongdoing scene examination for DNA and RNA sequencing. In numerous investigative studies to recognize obscure natural and inorganic mixes. Government research facilities used to weigh colors in nourishment and vegetables contained little measures of pesticides and herbicides. Types of Chromatography There are numerous types of chromatography, yet all structures take a shot at the same rule: Partition Chromatography which incorporates a fluid film covered in a dormant suitable backing. Adsorption Chromatography which incorporates finely separated robust working as an adsorbing surface they are partitioned finely to build their surface region. Ion Trade Chromatography (which is reversible step) which incorporates ionic gatherings (ionic means holding distinctive charges) which are joined to an inactive material; this technique is utilized within purging water for instance the opposition will be between the example (water considered portable stage additionally) the stationary stage specifically. gel Chromatography (additionally called sub-atomic sieving/Gel filtration/Gel penetration/Sub-atomic prohibition) which relies on upon a suspension of a polymer having a suitable pore size (like agar) is a vital strategy for a few examination sorts, for example, differentiating hormones, chemicals organic liquids; AGAR itself is a polymer with pores, so little particles will enter into the pores may leave just in the event that it discovered a bigger pore to enter in it Paper chromatography Paper chromatography is a logical strategy for differentiating and distinguishing both colored (e.g. colors) and boring (e.g. amino acids) mixtures. In paper chromatography, the stationary stage is an extremely uniform spongy paper. Cellulose (non polar) as paper sheets makes a perfect help medium where water is adsorbed to the cellulose filaments and structures the stationary hydrophilic stage. Cellulose is a polymer of the basic sugar, glucose. The key point about cellulose is that the polymer chains have -Goodness gatherings standing out all around them. The cellulose filaments draw in water vapor from the environment and in addition any water that was available when the paper was made. You can along these lines consider paper being cellulose strands with a meager layer of water particles bound to the surface. Non-polar particles in the mixture that you are attempting to particular will have little fascination for the water atoms joined to the cellulose, thus will invest a large portion of their time disintegrated in the moving dissolvable. Atoms like this will hence travel far up the paper conveyed by the dissolvable. They will have moderately high Rf values. Then again, polar particles will have a high fascination for the water atoms and significantly less for the non-polar dissolvable. They will consequently have a tendency to break down in the slim layer of water around the cellulose strands a great deal more than in the moving dissolvable. Objectives Identify the amino acids in unknown mixer, and calculate the Rf of each amino acids by paper chromatography. Materials Chromatography tank and lid Chromatography paper Capillary tubes Amino acid samples ( Arg, Trp, Tyr, Phe and unknown mixture of AA) Beaker BAW solvent – Butan-1-ol, acetic acid, and sterile water Ninhydrin Spray can Hair drier Latex gloves Methodology 10 Ãâ€" 20 centimeters chromatography paper was arranged by drawing a line 2 cm above from the bottom of the paper and little five imprints were made at comparable space interims alongside the line drawn. With particular slender tubes, arginine, phenylalanine, tyrosine, tryptophan and obscure amino corrosive mixture were tipped and were spotted on an imprint as they were named on the chromatograph paper, by touching the hairlike tubes on the chromatography paper and left them to dry and re-connected the examples a few times as the technique. After that chromatography paper was kept before the hair dryer to dry. When the BAW dissolvable of 100 ml was made by including Butan-1-ol, acidic corrosive, and sterile water in the 80:10:10 degrees. Fitting sum (around 1 cm) of BAW dissolvable was set into the chromatography tank. The chromatography paper was collapsed by the two side edges fit as a fiddle of barrel by stapling those edges close by without reaching each. After that the round and hollow chromatography paper was plunged equally into the BAW dissolvable and made to stand. Chromatography tank was shut by the cover and was left for 60 minutes. After that chromatography tank was revived and chromatography paper was expelled from the tank. The dissolvable line of the paper was checked right away and the chromatography paper was dried via hair drier. Following 05 minutes of drying, the entire paper was showered with ninhydrin by utilizing spread can. The spread paper was dried again utilizing hair drier. At last the separation went by specimen spots was stamped and separations were measured Results and Calculations The chromatogram Figure 01: The chromatogram The Rf calculated results Table 1. The results of Rf Sample Distance from star to Centre of spot (cm) Distance from start to solvent front Results / Rf Arginine 0.3 7.9 0.04 Tyrosine 1.9 7.9 0.24 Tryptophan 1.3 7.9 0.16 Phenylalanine 2 7.9 0.25 Unknown sample 0.3 7.9 0.04 1.1 7.9 0.13 Discussion In this analysis, pencil was utilized to draw the lines as opposed to pen in light of the fact that pen ink additionally moved by versatile stage and it may cause perusing mistake while taking the separation went by part from application point. At the point when setting the chromatography paper into chromatography tank the paper ought not to touch the divider of the tank, in the event that it does the paper will get polluted. The chromatogram is permitted to dry and is then showered with an answer of ninhydrin. Ninhydrin responds with amino acids to give colored mixes, primarily profound pink or purple shade. This chromatography system is likewise used to know the extremity of amino acids. In the event that an amino corrosive makes a high Rf worth means, that amino corrosive has less extremity; less extremity amino acids can without much of a stretch move towards the portable stage and shaped high Rf. High extremity amino acids dependably make less Rf esteem, it needs time to move towards the versatile stage. Wear gloves when taking care of Chromatography paper to abstain from touching the surface of the paper to be utilized for amino-corrosive chromatography in light of the fact that the skin contains amino acids the Chromatography paper as to abstain from saving amino acids from the fingers. On the off chance that it store aggravate the spots create. Regularly BAW dissolvable is use in Chromatography tank. It is a mixture segment of Butan-1-ol: Acetic corrosive: Water in a certain degree (60:15:25). Yet In this reasonable BAW mixture part of Butan-1-ol: Acetic corrosive: Water in a certain proportion (80:10:10) on the grounds that dissolvable of the BAW contain high measure of Butan-1-ol. It is cant break up in water. In this handy, utilized homogenous of the BAW result thusly change the degree of the contain parts. At the point when putting the chromatography paper into chromatography tank the paper was deliberately set ought not to touch the divider of the tank on the grounds that in the event that it touches the divider of the tank the paper will get defiled and the dissolvable ascend through the divider of the tank. The different amino acids are imperceptible. The acids might be envisioned by splashing the paper with a compound called ninhydrin. The chromatogram was permitted to dry then result of ninhydrin was spread on the chromatogram. Perception is Purple shade in light of the fact that ninhydrin responded with amino acids to give shaded mixes, essentially profound pink or violet color. Conclusion The Rf value of unknown mixture from this experiment was 0.04 and 0.13and it suggested the possibility to presence of two different amino acids such as arginine and tryptophan by having same Rf values (0.04 and 0.13). By the chromatography technique, identified the different amino acids in unknown mixers. References Ahuja, S. (2003) Chromatography and separation science, 1st ed, Amsterdam, Academic Press. , [Online] Available at: http://books.google.lk/books?id=KEynHdUwmFkCpg=PA101dq=chromatography+paper+techniqueshl=ensa=Xei=90rHU5_AHMTe8AXzmIGABQved=0CFAQ6AEwBw#v=onepageq=chromatography paper techniquesf=false (Accessed 03 September 2014). Banga, A. (2005) Therapeutic peptides and proteins, 1st ed, Boca Raton, Fla., CRC. , [Online] Available at: httphttp://books.google.lk/books?id=rPFw2iPB51gCdq=20+amino+acidssource=gbs_navlinks_s (Accessed 03 September 2014). Brian, M. (2000) Chromatography. [Online] Available at: http://www.files.chem.vt.edu/chem-ed/sep/chromato.html (Accessed 03 September 2014). Carraher, C. (2013) Introduction to polymer chemistry, 1st ed, Boca Raton, FL, CRC Press. , [Online] Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862331/ (Accessed 03 September 2014). Chawla, R. (n.d.) Practical clinical biochemistry, 1st ed. , [Online] Available at: http://books.google.lk/books?id=HP2YAwAAQBAJdq=chromatography+paper+techniquessource=gbs_navlinks_s (Accessed 03 September 2014). Clark, J. (2007) Chemguide.co.uk. [Online] Available at: http://www.chemguide.co.uk/analysis/chromatography/paper.html (Accessed 03 September 2014). Clark, J. (2007) Paper Chromatography. [Online] Available at:http://www.chemguide.co.uk/analysis/chromatography/paper.html (Accessed 03 September 2014). Collman, J. (2001) Naturally dangerous, 1st ed, Sausalito, CA, University Science Books. , [Online] Available at: http://books.google.lk/books?id=LM1I9zVPcXsCpg=PA9dq=20+amino+acidshl=ensa=Xei=EVXHU_KjLJPp8AXDkoLQDgved=0CD4Q6AEwBw#v=onepageq=20 amino acidsf=false (Accessed 03 September 2014). Dasgupta, A. (2010) Advances in chromatographic techniques for therapeutic drug monitoring, 1st ed, Boca Raton, CRC Press/Taylor Francis. , [Online] Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862331/ (Accessed 03 September 2014). Katoch, R. (2011) Analytical techniques in biochemistry and molecular biology, 1st ed, New York, Springer Science+Business Media, LLC. , [Online] Available at: http://books.google.lk/books?id=rCU2-gvkjo0Cpg=PA43dq=chromatography+paper+techniqueshl=ensa=Xei=90rHU5_AHMTe8AXzmIGABQved=0CDwQ6AEwBA#v=onepageq=chromatography paper techniquesf=false (Accessed 03 September 2014). Phytochemical analysis, 1st ed, Boca Raton, CRC Press. , [Online] Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862331/ (Accessed 03 September 2014). Trimpe, T. (2009) Chromatography basics. Sciencespot.net [e-book reader]. Available at: http://sciencespot.net/Media/FrnsScience/chromacard.pdf (Accessed 03 September 2014). Waksmundzka-Hajnos, M. and Sherma, J. (2011) High performance liquid chromatography in , [Online] Available at: http://books.google.lk/books?id=8F4LCU-AIhMCpg=PA14dq=chromatography+paper+techniqueshl=ensa=Xei=KUvHU5DrJsv_8QWp9ICIAQved=0CCgQ6AEwATgK#v=onepageq=chromatography paper techniquesf=false (Accessed 03 September 2014). Wilson, K. and Walker, J. (2000) Principles and techniques of practical biochemistry, 1st ed, Cambridge, Cambridge University Press. , [Online] Available at: http://books.google.lk/books?id=0NvlqobpwCwCpg=PA620dq=chromatography+paper+techniqueshl=ensa=Xei=KUvHU5DrJsv_8QWp9ICIAQved=0CCMQ6AEwADgK#v=onepageq=chromatography paper techniquesf=false (Accessed 03 September 2014).

Saturday, July 20, 2019

Free Essays on Whartons Ethan Frome: A Timeless Novel :: Ethan Frome Essays

Ethan Frome: A Timeless Novel  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚      The writing in the novel, Ethan Frome is fantastic. The love of Ethan Frome is crystal clear. Ethan and Mattie are both believably in love and Ethan's desperation grips the reader. Zeena, I think, is the most well described of them all. She is reality itself--beyond love, beyond fate, and it is she who outlasts them all. Although I think I fell in love with both Mattie and Ethan in this story and was feeling that intense love and pain of impending separation in their last moments together, the realist in me loved the ending! Zeena, the old witch, the nagging and cunning negative hag, is the one who is the rock in the moving stream. It's so 20th century. There is something black about the ending that you have to like. I like the way Zeena's image keeps popping up for Ethan: Zeena's empty rocking-chair stood facing him. Mattie rose obediently, and seated herself in it. As her young brown head detached itself against the patch-work cushion that habitually framed his wife's gaunt countenance, Ethan had a momentary shock. It was almost as if the other face, the face of the superseded woman, had obliterated that of the intruder. And as he's trying to enter into eternity with his beloved . . . But suddenly his wife's face, with twisted monstrous lineaments, thrust itself between him and his goal, and he made an instinctive movement to brush it aside. Here are some example of the accurate description that I love in this story: Through the obscurity which hid their faces their thoughts seemed to dart at each other like serpents shooting venom. Ethan was seized with horror of the scene and shame at his own share in it. It was as senseless and savage as a physical fight between two enemies in the darkness. All the long misery of his baffled past, of his youth of failure, hardship and vain effort, rose up in his soul in bitterness and seemed to take shape before him in the woman who at every turn had barred his way. She had taken everything else from him; and now she meant to take the one thing that made up for all the others. For a moment such a flame of hate rose in him that it ran down his arm and clenched his fist against her.

Friday, July 19, 2019

Kant Political Leader :: essays research papers

Kant held that nothing was good in itself except good will. In other words, no action, in and of itself, was either wrong or right. Only the motive of the actor lent the action its morality. If a person acted out of a vested interest (because of a possible consequence) then the act was non-moral—it had no moral implications whatsoever. But, if a person acted because she thought she was doing the right thing, then she was acting out of good will and the act was a moral act. In Kant’s view, actions have true moral worth only when they spring from a recognition of a duty and a choice to discharge it. For example, using Kantian logic, an advertiser who avoided untruthful advertising because he was afraid of getting caught and fined would not necessarily be acting morally. However, if the advertiser recognized a duty to his constituents to tell the truth, and that is the reason he didn't lie, then the act would be a moral act. Kant defined good will as the uniquely human capacity to act according to one's principles, not out of an expectation of potential consequences. In fact, Kant had learned through the writings of the Italian philosopher and royal counselor, Niccolo Machiavelli, that basing decisions solely on likely consequences could excuse any action, even the most abhorrent. In his famous treatise, The Prince, Machiavelli had proposed that any action taken by a monarch should be based on an assessment of the best outcome for the monarch himself. Under this guideline (which is also known as egoism), actions such as murder could be excused if they are in the best interest of the person making the decision. Like other Enlightenment theorists, Kant believed that human beings were endowed with the ability to reason, and reasoning would logically lead to an understanding of how to construct moral rules to live by. Rational beings would, then, logically abide by the rules they set for themselves. In this, he was in accord with the social contractarians. Rules arrived at in this manner would also become morally obligatory, and Kant saw obligation (or duty) as the overriding determinant of morality. He believed that we would recognize our duty when we saw it because we could reason, and reason would lead us logically to recognition. For Kant, there were two obvious types of duties: perfect duties and imperfect duties.

Thursday, July 18, 2019

Unit 12

Unit 12: Business Online Assignment Brief 2012-2013 Edexcel BTEC Level 2 in Business ————————————————- Date assignment issued: 1 March 2013 ————————————————- Task submission closed: 29 April 2013 Contents Scenario3 Task 14 Task 25 Task 36 Task 47 Task 58 Task 6:9 Task 710 Task 711 Task 812 Task 913 Task 1014 ScenarioYou are the winner of a Young Enterprise Award. You are pleased that the large cash prize will help you to start your own local business in the Croydon area.Your choice of business will be one of the following: * Luxury car hire * Designer bags * Cosmetics for men * Travel agents * Clothes for babies and small childrenYou will need to design a website for your business to include the following: * An online request system for your customers * Location o f the business * Products or services available| Task 1 Front cover Design a Front Cover for your assignment. It should include the name of your business a relevant image and your name. Introduction Outline the purpose of the assignment and introduce the business you will write about Business IntroductionIntroduce the business you will be writing about by writing a report to include: * Name of the business * Type of ownership (ie sole trader, private Ltd etc) * Product/service being sold * Proposed location * Who are your customers * Identify the resources and equipments you would require to get started Check you have done the following: * Used paragraphs to structure your work * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 11 March 2013 Time Deadline: 4pm Task 2 ————————————————-P6: D escribe the benefits to your business of marketing your product or service online Write a report that will describe 3 benefits to your business & 3 benefits to your customers of marketing your product or service online Check you have done the following: Used paragraphs to structure your work * Proof read your work using * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 11 March 2013 Time Deadline: 4pm Task 3 ————————————————- P1 Describe three different business organisations which operate online.Write a report using the following to help you: You must select a business from each of the following sectors: * Commercial * Educational * Government For each business you must also describe the purpose of their online presence you may use the following to help you: * Direct online selling of services * Government ser vices * Information * Advertising and marketing * Education Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document Upload this document to Moodle Task Deadline: 11 March 2013 Time Deadline: 4pm Task 4 ————————————————- M1 : Compare the features of three business organisations operating online Prepare a power point presentation comparing three business organisations operating online include the following: * Compare the features of each business * Explain how effective the features are for each organisation ————————————————- D1: Make at least three recommendations for a business organization considering go ing onlinePrepare a power point presentation giving three recommendations for a business organization considering going online Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline:25 March 2013 Time Deadline: 4pm Task 5 ————————————————- P2: Explain how each of the three businesses operate their activities online. ————————————————- Write a report using the following to help you: Brochure * Mail order * Inviting online transactions * Providing information only You must explain how each business use their website to engage and interact with users Check you h ave done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 18 March 2013 Time Deadline: 4pm Task 6: ————————————————- P3: Explain the issues your small business would need to consider to go onlineWrite a report to explain 3 issues your small business would need to consider to go online. You should investigate the functional areas behind an online presence and how these interact to support the website Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 25 March 2013 Time De adline: 4pm Task 7 ————————————————- P5: Create web pages to meet the needs of your customersCreating a web page Create web pages to meet the needs of your customers you must include some of the following: * Word, power point or other suitable packages * Hyperlinks, graphics, background colours, images * Images from scanners, digital cameras, clip art etc When you have finished your design you must check the: * colour combinations, hyperlinks are functioning, content, formatting, images etc M3: Explain how the website assists in achieving the aims and objectives of the business user| Write a report to explain how the website assists in achieving the aims and objectives of the business user.You must explain why marketing online fits in with the aims, objectives and operation of the organization. Task Deadline:15 April 2013 Time Deadline: 4pm Task 7 D3: Justify the use of di fferent features in the design of your website| Prepare a power point presentation to justify the use of different features in the design of your website and how it contributes to the success of the final creation. Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) Save the document * Upload this document to Moodle Task Deadline:15 April 2013 Time Deadline: 5. 00pm Task 8 ————————————————- P4: Explain the operational risks for your business organisation operating online Write a report to explain 3 operational risks for your business organization operating online and the problems caused with the lack of face to face interaction. M2: Analyse the benefits to business and customers of conducting business online| Write a report to ana lyse the benefits to business and customers of conducting business online.You should analyse the features of the web pages you have created; explain their suitability and how the online presence will benefit the business and its customers. D2: Suggest ways in which your business could deal with the operational risks associated with an online presence| Prepare a power point presentation suggesting ways in which your business could deal with the operational risks associated with an online presence. You must identify the risks and the methods a business could use to stop them. Check you have done the following: * Used paragraphs to structure your work, using your own words – not opied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 22 April 2013 Time Deadline: 4pm Task 9 P7 Outline the impact of online business on society| Write a report to outline the impact of online business on society. You should include the following: * Global presence * Level of response * Financial advantages * Effects on customer convenience Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 22 April 2013 Time Deadline: 4pm Task 10 M4: Analyse the consequences on society of an increase in online business| Write a report to analyse the consequences on society of an increase in online business. Use an example of an existing business you are familiar with to analyse how people’s lives have been affected. D4: Evaluate the benefits and drawbacks to society of increasing business online| Write a report to evaluate the benefits and drawback to society of increasing business online.You must include in your report the impact of going online both now and in the future. Check you have done the following: * Used paragraphs to structure your work, using your own words – not copied and pasted * Proof read your work * Run Spell checker and Grammar checker (F7) * Save the document * Upload this document to Moodle Task Deadline: 29 April 2012 Time Deadline: 4pm Upskills linkTasks 1-10 you will be required to judge how much to write and the level of detail to include. You must present information in a logical sequence using paragraphs where appropriate. Write in complete sentences, using correct grammar and spelling. |

Nathaniel Hawthorne’s as Anti-Transcendentalist Essay

Nathaniel Hawthorne an American romantic writer, whose belles-lettres is known for its Anti-Transcendentalism, wrote Young Goodman brownness and The cerise letter. Nathaniel Hawthorne is a writer during the American Romantics, where the majority of physical composition is foc theatrical roled on nature, supernatural ele custodyts wizs psychological science and the criticism of societys norm. Nathaniel is Hawthorne known for his nonorious Anti-Transcendentalist literary works. Anti-Transcendentalism ideals ar nearly ones abhorrence and intimate and sinful nature, where everyone is born condemnable and no matter what, and that human beingsity is evil and destructive. Hawthorne is non able to accept the optimism of the Transcendentalists and through his make-up he tries to look for truths in ones heart and mind. Hawthornes use of symbols, characters, and themes in his belles-lettres distinguish his publications as Anti-Transcendentalist.Through the use of character knowledge Hawthornes literature can be assort as Anti-Transcendentalist.In two(prenominal) pieces of literature Hawthorne uses characters who act as the image of Anti-Transcendentalism. The carmine garner has two compulsive characters that exemplify Anti-Transcendentalist. First we have Dimmesdale he is a minister in the townsfolk and is one of the some highly see men in Boston. In ingenuousness Dimmesdale is a s versed and an adulterer as he slept with Hester, another mans wife and got her pregnant. Hesters punishment for her actions argon public humiliation, and the Scarlet Letter. Though Dimmesdale is not revealed as her accomplice over date he himself grows a Scarlet Letter of his own. Most of the spectators testified to having seen, on the breast of the infelicitous minister, a Scarlet LetterMr. Dimmesdale, on the very day when Hester Prynne first wore her bootleg badge, had begun a course of penance(SL 211). When Hawthorne reveals Dimmesdale having a Scarlet Lett er it expresses the ideals of Anti-Transcendentalism where his own Scarlet Letter is the example of ones home(a) darkness and evil that is interior of him.In Young Goodman brown afterwards Goodman cook witnesses all of the townsthe great unwashed take part in a witchcraft eucharist and comes back to his home he sees the cosmealy concern in a new perspective. The undermentioned morning Goodman Brown came slowly into the streets of capital of Oregon staring near him like a bewildered man(YGB 7). He is neer the same and trusts no one, including his wife. It says that he snatched forth the boorren that Goody Cloyse was talking to and when his wife embraced him he just walked away.(YGB 7 )Hawthorne uses Goodman Brown to study that everyone is evil inside(a) and that he human drift is destructive. Character development acknowledges a role in how Hawthornes literature should be classified as Anti-Transcendentalist.Character development is not the lone(prenominal) factor t hat classifies Hawthornes literature as Anti-Transcendentalist symbols take on an enormous role as well. In the Scarlet Letter two symbols be pearl, and the actual Scarlet Letter. cliff is not only a peculiar child for her age she has a much deeper meaning. Pearl is Hesters living example of her sin. Pearls purpose in the Scarlet Letter is to represent sin, but not only to Hester but in the world as well. The Actual Scarlet Letter is as well as a symbol which represents not only sin, but the Anti-Transcendentalist idea that your sins testament never go away, sin give remain evident inside you and around you. These two examples could not exemplify Anti-Transcendentalist beliefs some(prenominal) more as its primary counseling is about sinfulness and how people escort sin. In Young Goodman Brown a few symbols that embody Anti-Transcendentalism atomic number 18 the tone and the anile man who is meant to be the remonstrate.The forest in both of Hawthornes literature has a d ark meaning towards it and also is a place where no well-behaved can happen Goodman Brown believes that the trouble could be present in the forest, and when he sees a woman from his town he hides as he is ashamed to be walking in the forest. The forest is characterized as devilish, frightening, and dark. The old man is also a symbol which is portrayed as the devil. He is used to represent the destructiveness of the human spirit and to generate the evil within human beings. all(prenominal) of these characteristics contribute to classify Hawthornes writing as Anti-Transcendentalist.Aside from characters and symbols Hawthorne uses themes in his literature which contribute to classifying his writings as Anti-Transcendentalist. The around prominent theme in both pieces of his literature is the, nature of evil in humanity. The Scarlet Letter uses the Black art object as an alter ego to Chillingworth who is a representation for the Devil. On the outside Chillingworth is a doctor who is supposed to help people when they be sick but inside he is evil. Instead of giving Dimmesdale lifespan he is making him sick, as he is fetching life and making it miserable. take away, or yonder old down(p) man will duck soup you He hath got hold of the minister already. Come away, have or he will catch you(SL 61) The quote is from Pearl who understands Chillingworth is the devil and has already captured and made Dimmesdales life horrible. another(prenominal) instance of the evil in humanity partakes in the Story of Young Goodman Brown where it contains a greater depth of evil in humanity.The Scarlet Letter is only one man consumed by the devil but in Young Goodman Brown the whole town appears to be taking part in a witchcraft ceremony. When he is taken into the forest he sees the minister, a deacon and many more respected men of his town are evil. He recognized a cook of church members of SalemGood old Deacon Gookin had arrived, his reserved pastor(YGB 6) These men and women who are highly respected are taking part in side witchcraft. Hawthorne is expressing that humans are capable of inner darkness and evil, which enable his literature to be classified as Anti-Transcendentalist. Symbols, characters, and themes are the elements that Hawthorne uses to show how his literature can be classified as Anti-Transcendentalist.The symbols in both pieces of Hawthornes literature represent sin, and evil, both which are qualities of Anti-Transcendentalism. All the characters in Hawthornes literature contain evidence of sinful nature, inner darkness and the destructiveness of the human spirit. These qualities are most prominent in Chillingworth and Dimmesdale and especially with Goodman Brown. last Hawthornes primary theme in all his literature is the nature of evil in humanity. Chillingworth exemplifies this theme greatly as well as the towns people in Young Goodman Brown. The way Hawthorne writes and his use of symbols, themes, and characters classify his wr iting as Anti-Transcendentalist.